Synthesis and Fluorescence Property of the GFP Chromophores’ Derivatives
YE Jian-heng1,2, WANG Chao1, DI Xiao1, SUN Jian1, TANG Zhuo1
1. Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China
Abstract：Eight novel analogs(5a~5h) of fluorescent protein chromophore were designed and synthesized by condensation between aromatic aldehydes and imidazolinone, which were obtained by acetylation, substitution and Wittiong reaction, starting from N-methylacetamide or N-methylbenzamide. The structures were characterized by 1H NMR and 13C NMR. The optical study showed that the fluorescent emission wavelengths of most compounds were red-shifted compared with p-hydroxybenzylideneimidazolinone. The emission wavelength of (Z)-4-(2-hydroxy-5-nitrobenzylidene)-1-methyl-2-phenyl-1H-imidazol-5(4H)-one (5f) was 614 nm.
叶剑衡， 王超， 底霄， 孙健， 唐卓. GFP生色团衍生物的合成及其荧光性能[J]. 合成化学, 2016, 24(3): 231-234.
YE Jian-heng, WANG Chao, DI Xiao, SUN Jian, TANG Zhuo. Synthesis and Fluorescence Property of the GFP Chromophores’ Derivatives. Chinese Journal of Synthetic Chemistry, 2016, 24(3): 231-234.
2016, Vol. 24 
