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| Synthesis and Fluorescence Property of the GFP Chromophores’ Derivatives |
| YE Jian-heng1,2, WANG Chao1, DI Xiao1, SUN Jian1, TANG Zhuo1 |
| 1. Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China |
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Abstract Eight novel analogs(5a~5h) of fluorescent protein chromophore were designed and synthesized by condensation between aromatic aldehydes and imidazolinone, which were obtained by acetylation, substitution and Wittiong reaction, starting from N-methylacetamide or N-methylbenzamide. The structures were characterized by 1H NMR and 13C NMR. The optical study showed that the fluorescent emission wavelengths of most compounds were red-shifted compared with p-hydroxybenzylideneimidazolinone. The emission wavelength of (Z)-4-(2-hydroxy-5-nitrobenzylidene)-1-methyl-2-phenyl-1H-imidazol-5(4H)-one (5f) was 614 nm.
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Received: 16 April 2015
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2016, Vol. 24 
